Palmityl coenzyme A deacylase.

Several different enzymes have been described which catalyze hydrolysis of acyl CoA derivatives. Acetyl CoA deacylase z, and succinyl CoA deacylase z-4, hydroxyisobutyryl CoA deaeylase ~ and a hydroxymethyl glutaryl CoA deacylase 6 have been described. Enzyme preparations that deacylate acetoacetyl CoA have been described~, 8 but recent evidence points to a more involved mechanism than one of simple hydrolysis 9, lO In addition, cleavage of long chain fatty acyl glutathione compounds have been reported u, 13. PORTER AND TIETZ and PORTER AND LONG have reported the presence of palmityl CoA deacylase ill pigeon liver 13,14. The purpose of this paper is to present an isolation procedure and several assay methods for a long chain fatty acyl CoA deacylase found in pig brain.